Cohen Lab

Physical tools to study molecules, cells, and organisms

We invent new physical tools to probe biological structures. We use our tools to make new measurements. We choose problems by looking in unexplored regimes of time and space. We combine protein engineering, lasers, nanofabrication, microfluidics, electronics, biochemistry, and computers to generate data; and we apply statistics and physical modeling to understand the data.

 

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Optically triggered action potential in a rat hippocampal neuron, mapped at 100,000 frames/s.

 

Research Areas

 

Previous projects

Optical chirality, trapping single molecules, microbial and molecular motion in mucus, nonequilibrium van der Waals forces, DNA mechanics, spectral shift FRET, science education in Liberia.

 

News

 

Poster children (7/2018)

Two labmembers won 'Best Poster' awards at separate conferences this summer. Congratulations to Zheng for winning at the FASEB conference on Molecular Biophysics of Membranes. Congratulations to Yoav for winning at the Gordon Research Conference on Optogenetics and Imaging. Hooray!

Cell membranes resist flow (5/2018)

Zheng's paper is on the bioRxiv! Cell membranes are more like Jello than maple syrup. This was a surprise to us--maybe to you too!

All-optical electrophysiology in awake, behaving, mice (3/2018)

Yoav's paper is on the bioRxiv! This heroic piece of work combines new voltage indicators, new microscopes, new mice, and new software. Great team effort! All to see how the hippocampus represents locomotion.

Welcome Undergrads (2/2018)

Welcome to Yooree Ha and Rajath Salegame. Yooree is working with Urs on voltage imaging in zebrafish vasculature, and Rajath is working with Harry on bioelectrical phase transitions. Welcome aboard!

Welcome Shane (1/2018)

Shane Nichols joins the lab as a new postdoc. He comes from the lab of Bart Kahr where he studied chirality. Shane will be developing a new twist on brain imaging!

Ultrawide-field microscope (12/2017)

Kit's super wide-area bidirectional microscope (for imaging and stimulation) comes out in Biomedical Optics Express. Nice job, Kit!

Some press here: Science Daily, Harvard's Facebook page.

Two-photon photo-activated voltage indicators (11/2017)

Miao and Daan developed NovArch, a new type of voltage indicator which shows two-photon sensitized one-photon fluorescence. Super useful for dendritic voltage imaging in acute slices. Check out the preprint on the bioRxiv.

The voltage indicator family tree (8/2017)

In this review article with Peng Zou's group we try to trace the family tree of voltage indicators, and to predict where new branches will blossom. See the paper in Current Opinion in Chemical Biology.

Optogenetics in drug discovery (7/2017)

Hongkang and I discuss the myriad ways bidirectional optical control can be used to enhance drug discovery. Check it out in Trends in Biotechnology!

Farewell to Daan, Miao, and Xin (3/2017)

Three postdocs are departing to start their own labs. Daan is starting his lab at Delft University of Technology, and Miao is starting hers at Erasmus University Medical Center, Rotterdam. Xin is starting at University of Florida. Good luck to all!

Newest lab member (2/2017)

Welcome to Lionel An-Ren Cohen! He is starting to work on data analysis.

Be still, my heart (2/2017)

Kit and Miao's work on optical electrophysiology of hiPSC-derived cardiomyocytes just came out in PLoS One. They combined patterned cell growth, optical electrophysiology and transcriptomics to identify geometry-dependent changes in cardiomyocyte function and gene expression.

The real-time technicolour living brain (11/2016)

news story in Nature describe A news story in Nature describes recent advances in optical probing of neural activity. Check out some vignettes of our work and beautiful photos from Tian and Sami!

Take a virtual lab tour (10/2016)

Join Sami and Adam for a virtual lab tour on Harvard's Facebook Live site. We deal with lots of neuron culture media, but this is our lab's first foray into social media!

Painting with Rainbows (10/2016)

We wrote a paper describing the amazing optogenetic tricks one can play with spatially, temporally, and spectrally sculpted light. Read about it in Accounts of Chemical Research.

Those blinking mice (10/2016)

Shan developed a transgenic Cre-dependent Optopatch mouse, enabling genetically targeted all-optical electrophysiology in intact tissue. And it's on the cover of Journal of Neuroscience!

Taking our cells for a spin (6/2016)

Harry engineered bioelectric circuits from scratch! Read about optically controlled cellular ring oscillators and other adventures in synthetic neurophysiology in PRX. See also a news story in Physics World.

Spiking HEK cells for ion channel screening (6/2016)

Hongkang's paper showing an all-optical approach for studying ion channel electrophysiology just came out in eLife. Who knew NaV1.7 could be so much fun!

Biophysics Week Review (3/2016)

Did you remember to celebrate Biophysics Week? In honor of the occasion, Adam wrote a review on Optogenetics, which became the cover story in Biophysical Journal.

A FLICR in the Dark (2/2016)

Robert Campbell's lab developed a red-shifted voltage indicator, and we got to help! Read about in Journal of Neuroscience.

 

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©2015 Adam E. Cohen