Cohen Lab

Articles:

  1. C. Scheibner, H. Ori, A. E. Cohen, V. Vitelli,  Spiking at the edge: Excitability at interfaces in reaction–diffusion systems, PNAS 121, e2307996120, 2024. See also BioRxiv preprint.
  2.  P. W. Liu, H. Zhang, C. A. Werley, M. Pichler, S. J. Ryan, C. L. Lewarch, J. Jacques, J. Grooms, J. Ferrante, G. Li, D. Zhang, N. Bermmer, A. Barnett, R. Chantre, A. Elder, A. E. Cohen, L. A. Williams, G. T. Dempsey, O. B. McManus,  A phenotypic screening platform for chronic pain therapeutics using all-optical electrophysiology, Pain, 2023.
  3. S. Kim, G. Ko, I. Kang, H. Tian, L. Z. Fan, Y. Li, A. E. Cohen, J. Wu, Q. Dai, M. Choi,  Optical segmentation-based compressed readout of neuronal voltage dynamics, BioRxiv, 2023
  4. H. C. Davis, F. P. Brooks III, J. D. Wong-Campos, A. E. Cohen, Optical constraints on two-photon voltage imaging, BioRxiv, 2023. 
  5. K. M. Xiang, P. Park, S. A. Koren, R. Frank Hayward, A. E. Cohen, All-optical mapping of cAMP transport reveals rules of sub-cellular localization, BioRxiv 2023.
  6. D. Kim, P. Park, X. Li, J.D. Wong-Campos, H. Tian, E. M. Moult, J. B. Grimm, L. Lavis, A. E. Cohen, Mapping memories: pulse-chase labeling reveals AMPA receptor dynamics during memory formation, BioRxiv, 2023.
  7. P. Park*, J.D. Wong-Campos*, D. G. Itkis, Y. Qi, H. Davis, J. B. Grimm, S. E. Plutkis, L. Lavis, A. E. Cohen, Dendritic voltage imaging reveals biophysical basis of associative plasticity rules, BioRxiv, 2023.
  8. J.D. Wong-Campos*, P. Park*, H. Davis, Y. Qi, H. Tian, D. G. Itkis, D. Kim, J. B. Grimm, S. E. Plutkis, L. Lavis, A. E. Cohen, Voltage dynamics of dendritic integration and back-propagation in vivo, BioRxiv, 2023
  9. R. Frank Hayward, F. P. Brooks III, S. Yang, S. Gao, A. E. Cohen, Diminishing neuronal acidification by channelrhodopsins with low proton conduction, eLife 12:RP86833, 2023.
  10. M. Eom, S. Han, G Kim, E. S. Cho, J. Sim, P. Park, K. H. Lee, S. Kim, M. Rozsa, K. Svoboda, M. Choi, C. H. Kim, A. E. Cohen, J. B Chang, Y. G. Yoon, Statistically unbiased prediction enables accurate denoising of voltage imaging data ,Nature Methods, 20, 1581-1592, 2023. Cover story.
  11. B. Jia, Y. Qi, J. D. Wong-Campos, S. Megason, A. E. Cohen, A bioelectrical phase transition patterns the first vertebrate heartbeats, Nature, 622, 149-155, 2023.  See also BioRxiv preprint, 2023.
  12. L. Z. Fan, D. K. Kim, J. H. Jennings, H. Tian, S. Randles, C. Ramakrishnan, Y. Sun, E. Thadhani, Y. S. Kim, P. Y. Wang, S. Quirin, L. Giocomo, A. E. Cohen, K. Deisseroth, All-optical physiology reveals a synaptic basis for behavioral time scale plasticity, Cell, 186, 543-559, 2023.
  13. H. Tian, H. C. Davis, J. D. Wong-Campos, L. Z. Fan, B. Gmeiner, S. Begum, C. A. Werley, G. B. Borja, H. Upadhyay, H. Shah, J. Jacques, P. Park, Y. Qi, V. Parot, K. Deisseroth, A. E. Cohen, Video-based pooled screening yields improved far-red genetically encoded voltage indicators, Nature Methods, 20, 1082–1094, 2023. See also Biorxiv preprint for more details on interneuron dynamics.
  14. D. Lin, X. Li, E. Moult, P. Park, B. Tang, H. Shen, J. B. Grimm, N. Falco, B. Jia, D. Baker, L. D. Lavis, A. E. Cohen, Time-tagged ticker tapes for intracellular recordings, Nature Biotechnology, 41, 631-639, 2023.
  15. H. Ori, M. Duque Ramirez, C. Scheibner, V. Vitelli, A. E. Cohen, Observation of topological action potentials in engineered tissues, Nature Physics 19, 290-296, 2023.
  16. Z. Shi, S. Innes-Gold, and A. E. Cohen, Membrane tension propagation couples axon growth and collateral branching, Science Advances 8.35, eabo1297, 2022.
  17. M. Armbruster, S. Naskar, J. P. Garcia, M. Sommer, E. Kim, Y. Adam, P. G. Haydon, E. S. Boyden, A. E. Cohen, and C. G. Dulla, Neuronal activity drives pathway-specific depolarization of peripheral astrocyte processes, Nature Neuroscience 25, 607-616, 2022
  18. A. T. Landau, P. Park, J. D. Wong-Campos, H. Tian, A. E. Cohen, B. Sabatini, Dendritic branch structure compartmentalizes voltage-dependent calcium influx in cortical layer 2/3 pyramidal cells, eLife 11:e76993, 2022. https://doi.org/10.7554/eLife.76993
  19. U. L. Böhm, Y. Kimura, T. Kawashima, M. B. Ahrens, S.I. Higashijima, F. Engert, A. E. Cohen, Voltage imaging identifies spinal circuits that modulate locomotor adaptation in zebrafish, Neuron 110, 1-12, 2022.
  20. J.D. Wong-Campos, J. V. Porto, A. E. Cohen, Which way does stimulated emission go?, J. Phys. Chem. A 125, 10667-10676, 2021.
  21. W. Bloxham, D. Brinks, S. Kheifets, A. E. Cohen, Linearly polarized excitation enhances signals from fluorescent voltage indicators, Biophys. J. 120, 5333-5342, 2021.
  22. M.-P. Chien*, D. Brinks*, G. Testa-Silva, H. Tian, F. P. Brooks III, Y. Adam, W. Bloxham, B. Gmeiner, S. Kheifets, A. E. Cohen, Photoactivated voltage imaging in tissue with an archaerhodopsin-derived reporter, Science Advances 7, eabe3216, 2021. See also bioRxiv, 211946, 2017.
  23. Z. Al Tanoury, J. F. Zimmerman, J. Rao, D. Sieiro, H. M. McNamara, T. Cherrier, A. Rodriguez-de la Rosa, A. Hick-Colin, F. Bousson, C. Fugier-Schmucker, F. Marchiano, B. Habermann, J. Chal, A. P. Nesmith, S. Gapon, E. Wagner, V. A. Gupta, R. Bassel-Duby, E. N. Olson, A. E. Cohen, K. K. Parker, and O. Pourquie, Prednisolone rescues Duchenne Muscular Dystrophy phenotypes in human pluripotent stem cells-derived skeletal muscle in vitro, PNAS 118.28, 2021.
  24. M. E. Xie, Y. Adam, L. Z. Fan, U. L. Böhm, I. Kinsella, D. Zhou, M. Rozsa, A. Sing, K. Svoboda, L. Paninski, A. E. Cohen, High-fidelity estimates of spikes and subthreshold waveforms from 1-photon voltage imaging in vivo, Cell Reports 35, 108954, 2021.
  25. C.A. Werley, S. Boccardo, A. Rigamonti, E.M. Hansson, A.E. Cohen, Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology, Nature Communications, 11, 3881, 2020
  26. L.Z. Fan, S. Kheifets, U.L. Boehm, H. Wu, K.D. Piatkevich, M.E. Xie, V. Parot, Y. Ha, K.E. Evans, E.S. Boyden, A.E. Takesian, A.E. Cohen, All-Optical Electrophysiology Reveals the Role of Lateral Inhibition in Sensory Processing in Cortical Layer 1, Cell 180, 521-535.e18  2020.

    All-Optical Electrophysiology Reveals the Role of Lateral Inhibition in Sensory Processing in Cortical Layer 1

    Cortical layer 1 (L1) interneurons have been proposed as a hub for attentional modulation of underlying cortex, but the transformations that this circuit implements are not known. We combined genetically targeted voltage imaging with optogenetic activation and silencing to study the mechanisms underlying sensory processing in mouse barrel cortex L1. Whisker stimuli evoked precisely timed single spikes in L1 interneurons, followed by strong lateral inhibition. A mild aversive stimulus activated cholinergic inputs and evoked a bimodal distribution of spiking responses in L1. A simple conductance-based model that only contained lateral inhibition within L1 recapitulated the sensory responses and the winner-takes-all cholinergic responses, and the model correctly predicted that the network would function as a spatial and temporal high-pass filter for excitatory inputs. Our results demonstrate that all-optical electrophysiology can reveal basic principles of neural circuit function in vivo and suggest an intuitive picture for how L1 transforms sensory and modulatory inputs.

  27. H.M. McNamara, R. Salegame, Z. Al Tanoury, H. Xu, S. Begum, G. Ortiz, O. Pourquie, A.E. Cohen Bioelectrical Domain Walls in Homogeneous Tissues, Nature Physics, 16, 357-364, 2020.

    Bioelectrical Domain Walls in Homogeneous Tissues

    Electrical signalling in biology is typically associated with action potentials—transient spikes in membrane voltage that return to baseline. Hodgkin–Huxley and related conductance-based models of electrophysiology belong to a more general class of reaction–diffusion equations that could, in principle, support the spontaneous emergence of patterns of membrane voltage that are stable in time but structured in space. Here, we show theoretically and experimentally that homogeneous or nearly homogeneous tissues can undergo spontaneous spatial symmetry breaking through a purely electrophysiological mechanism, leading to the formation of domains with different resting potentials separated by stable bioelectrical domain walls. Transitions from one resting potential to another can occur through long-range migration of these domain walls. We map bioelectrical domain wall motion using all-optical electrophysiology in an engineered cell line and in human induced pluripotent stem cell (iPSC)-derived myoblasts. Bioelectrical domain wall migration may occur during embryonic development and during physiological signalling processes in polarized tissues. These results demonstrate that nominally homogeneous tissues can undergo spontaneous bioelectrical spatial symmetry breaking.

  28. A.E. Cohen, Z. Shi Do Cell Membranes Flow Like Honey or Jiggle Like Jello?, BioEssays, 42, 1900142, 2020.

    Do Cell Membranes Flow Like Honey or Jiggle Like Jello?

    Cell membranes experience frequent stretching and poking: from cytoskeletal elements, from osmotic imbalances, from fusion and budding of vesicles, and from forces from the outside. Are the ensuing changes in membrane tension localized near the site of perturbation, or do these changes propagate rapidly through the membrane to distant parts of the cell, perhaps as a mechanical mechanism of long-range signaling? Literature statements on the timescale for membrane tension to equilibrate across a cell vary by a factor of ≈106.This study reviews and discusses how apparently contradictory findings on tension propagation in cells can be evaluated in the context of 2D hydrodynamics and poroelasticity. Localization of tension in the cell membrane is likely critical in governing how membrane forces gate ion channels, set the subcellular distribution of vesicle fusion, and regulate the dynamics of cytoskeletal growth. Furthermore, in this study, it is proposed that cells can actively regulate the degree to which membrane tension propagates by modulating the density and arrangement of immobile transmembrane proteins. Also see the video abstract here https://youtu.be/T6K7AIAqqBs."

  29. Y. Adam, J.J. Kim, S. Lou, Y. Zhao, M.E. Xie, D. Brinks, H. Wu, M.A. Mostajo-Radji, S. Kheifets, V. Parot, S. Chettih, K.J. Williams, B. Gmeiner, S.L. Farhi, L. Madisen, E.K. Buchanan, I. Kinsella, D. Zhou, L. Paninski, C.D. Harvey, H. Zeng, P. Arlotta, R.E. Campbell, A.E. Cohen, Voltage imaging and optogenetics reveal behaviour-dependent changes in hippocampal dynamics , Nature, 569, 413-417, 2019.
  30. S.L. Farhi, V.J. Parot, A. Grama, M. Yamagata, A.S. Abdelfattah, Y. Adam, S. Lou, J. J. Kim, R.E. Campbell, D.D. Cox, A.E. Cohen, Wide-area all-optical neurophysiology in acute brain slices, The Journal of Neuroscience, 10, 0168-19, 2019.
  31. V. J. Parot, C. Sing-Long, Y. Adam, U. L. Bohm, L. Z. Fan, S. L. Farhi, A.E. Cohen, Compressed Hadamard microscopy for high-speed optically sectioned neuronal activity recordings, J. Phys. D: Appl. Phys., 52, 144001, 2019.
  32. Z. Shi, Z.T. Graber, T. Baumgart, H.A. Stone, A.E. Cohen, Cell membranes resist flow, Cell, 175, 1-11, 2018.
  33. H.M. McNamara, S. Dodson, Y. Huang, E.W. Miller, B. Sandstede, A.E. Cohen, Geometry-dependent arrhythmias in electrically excitable tissues, Cell Systems, 7, 359-370, 2018.
  34. L.Z. Fan, R. Nehme, Y. Adam, E.S. Jung, H. Wu, K. Eggan, D.B. Arnold, A.E. Cohen, All-optical synaptic electrophysiology probes mechanism of ketamine-induced disinhibition, Nature Methods, 15, 823-831, 2018. Supplementary Information.
  35. E. Kiskinis, J.M. Kralj, P. Zou, E.N. Weinstein, H. Zhang, K. Tisoras, O. Wiskow, J.A. Ortega, K. Eggan, A.E. Cohen, All-optical electrophysiology for high-throughput functional characterization of a human iPSC-derived motor neuron model of ALS, Stem Cell Reports, 10, 1-14, 2018.
  36. A.E. Cohen and S.L. Farhi, Sculpting light to reveal brain function, Nature Neuroscience, 21.6, 776, 2018.
  37. A.H. Squires, A.E. Cohen, W.E. Moerner, Anti-Brownian Traps, Encyclopedia of Biophysics, G. C. K. Robers, A. Watts (eds.)
  38. C.A.Werley, M.P.Chien, A.E.Cohen, Ultrawidefield microscope for high-speed fluorescence imaging and targeted optogenetic stiumation, Biomedical Optics Express, 8, 5794-5813, 2017.
  39. H.Zhang, A.E. Cohen, Optogenetic approaches to drug discovery in neuroscience and beyond, Trends in Biotechnology, 35, 625-639, 2017.
  40. Y.Xu, P. Zou, A.E. Cohen, Voltage imaging with genetically encoded indicators, Current Opinion in Chemical Biology, 39, 1-10, 2017.
  41. C.A. Werley, M.P. Chien, J. Gaublomme, K. Shekhar, V. Butty, B.A. Yi, J.M. Kralj, W. Bloxham, L.A. Boyer, A. Regev, A.E. Cohen, Geometry-dependent functional changes in iPSC-derived cardiomyocytes probed by functional imaging and RNA sequencing, PloS one, 12.3, e0172671, 2017.
  42. K. Ogden, W. Chen, S.A. Swanger, M.J. McDaniel, L.Z.Fan, C. Hu, A. Tankovic, H. Kusumoto, G.J. Kosobucki, A.J. Schulien, Z. Su, J. Pecha, S. Bhattacharya, S. Petrovski, A.E. Cohen, E. Aizenman, S.F. Traynelis, H. Yuan, Molecular mechanism of disease-associated mutations in the pre-M1 helix of NMDA receptors and potential rescue pharmacology, PLoS Genetics, 13, e1006536, 2017.
  43. D. Brinks, Y. Adam, S. Kheifets, A.E. Cohen, Painting with rainbows: patterning light in space, time, and wavelength for multiphoton optogenetic sensing and control, Accounts of Chemical Research, 49, 2518-2526, 2016.
  44. S. Lou, Y. Adam, E.N. Weinstein, E.Williams, K. Williams, V. Parot, N. Kavokine, S. Liberles, L. Madisen, H. Zeng, A.E. Cohen, Genetically targeted all-optical electrophysiology with a transgenic Cre-dependent Optopatch mouse, The Journal of Neuroscience, 43, 11059-11073, 2016.
    • Supplementary Information here.
    • Animal request form at JAX here.
  45. Y.K. Cho, G. Zheng, G.J. Augustine, D.R. Hochbaum, A.E. Cohen, T. Knöpfel, F. Pisanello, F.S. Pavone, I.M. Vellekoop, M.J. Booth, S. Hu, J. Zhu, Z. Chen, Y. Hoshi, Roadmap on neurophotonics, Journal of Optics, 18, 093007, 2016.
  46. H.McNamara, H. Zhang, C.A. Werley, A.E. Cohen, Optically controlled oscillators in an engineered bioelectric tissue, Phys. Rev., X 6, 031001, 2016. Supplementary material.
  47. H.Zhang, E.Reichert, A.E. Cohen, Optical electrophysiology for probing function and pharmacology of voltage-gated ion channels, eLife, 5, e15202, 2016.
  48. G. T. Dempsey, K.W. Chaudhary, N. Atwater, C. Nguyen, B.S. Brown, J.D. McNeish, A.E. Cohen, J.M. Kralj, Cardiotoxicity screening with simultaneous optogenetic pacing, voltage imaging and calcium imaging, Journal of Pharmacological and Toxicological Methods, 2016.
  49. A.E. Cohen, Optogenetics: turning the microscope on its head, Biophysical Journal, 110.5, 997, 2016.
  50. A. S. Abdelfatta, S. L. Farhi, Y. Zhao, D. Brinks, P. Zou, A. Ruangkittisakul, I. Platisa, V. A. Pieribone, K. Ballanyi, A. E. Cohen, R. E. Campbell, A bright and fast red fluorescent protein voltage indicator that reports neuronal activity in organotypic brain slices, The Journal of Neuroscience, 36.8, 2458-2472, 2016.
  51. M.J. Shon, A.E. Cohen, Nano-mechanical measurements of protein-DNA interactions with a silicon nitride pulley, Nucl. Acids Res., 44, e7, 2016.
  52. M.J. Henderson, H.A. Baldwin, C.A. Werley, S. Boccardo, L.R. Whitaker, X. Yan, G.T. Holt, E.R. Schreiter, L.L. Looger, A.E. Cohen, D.S. Kim, B.K. Harvey, A low affinity GCaMP3 variant (GCaMPer) for imaging the endoplasmic reticulum calcium store, PLoS One, 10.10, e0139273, 2015.
  53. V. Emiliani, A. E. Cohen, K. Deisseroth, M. Hausser, All-optical interrogation of neural circuits, J. Neurosci., 35.41, 13917-13926, 2015.
  54. H. Lee, D. Brinks, A.E. Cohen, Two-photon imaging of a magneto-fluorescent indicator for 3D optical magnetometry, Optics Express, 23.21, 28022-28030, 2015.
  55. D. Brinks, A.J. Klein, A.E. Cohen, Two-photon lifetime imaging of voltage indicating proteins as a probe of absolute membrane voltage, Biophysical Journal, 109, 914-921, 2015.
  56. M.P. Chien, C.A. Werley, S.L. Farhi, A.E. Cohen, Photostick: a method for selective isolation of target cells from culture, Chemical Science, 6, 1701-1705, 2015.
  57. V. Venkatachalam, A.E. Cohen, Imaging GFP-based reporters in neurons with multiwavelength optogenetic control, Biophys. J., 107, 1554-1563, 2014.
  58. D. Yang, A.E. Cohen, Chirality-dependent friction of bulk molecular solids, Langmuir, 30, 9972- 9976, 2014.
  59. J. Hou, J.M. Kralj, A.D. Douglass, F. Engert, A.E. Cohen, Simultaneous mapping of membrane voltage and calcium in zebrafish heart in vivo reveals chamber-specific development transitions in ionic currents, Front. Physiol., 5, 344, 2014. Supporting videos.
  60. P. Zou*, Y. Zhao*, A.D. Doughlass, D.R. Hochbaum, D. Brinks, C.A. Werley, D.J. Harrison, R.E. Campbell, A.E. Cohen (*Co-first authors), Bright and fast multicoloured voltage reporters via electrochromic FRET, Nature Communications, 5, 2014.
  61. D.R. Hochbaum*, Y. Zhao*, S.L. Farhi, N. Klapoetke, C.A. Werley, V. Kapoor, P. Zou, J.M. Kralj, D. Maclaurin, N. Smedemark-Margulies, J.L. Saulnier, G.L. Boulting, C. Straub, Y.K. Cho, M. Melkonian, G.K-S. Wong, D.J. Harrison, V.N. Murthy, B.L. Sebatini, E.S. Boyden, R.E. Campbell, A.E. Cohen (*Co-first authors), All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins, Nature Methods, 11, 825-833, 2014.
  62. A.E. Cohen, V. Venkatachalam, Bringing bioelectricity to light, Annu. Rev.Biophys., 43, 211-232, 2014.
  63. J. Hou, V. Venkatachalam, A.E. Cohen, Temporal dynamics of microbial rhodopsin fluorescence reports absolute membrane voltage, Biophysical Journal, 106.3, 639-648, 2014.
  64. V. Venkatachalam, D. Brinks, D. Maclaurin, D. Hochbaum, J.M. Kralj, A.E. Cohen, Flash memory: photochemical imprinting of neuronal action potentials onto a microbial rhodopsin, J. Am. Chem. Soci., 136, 2529-2537 2013.
  65. J. Park , C.A. Werley, V. Venkatachalam, J.M. Kralj, S.D. Dib-Hajj, S.G. Waxman, A.E. Cohen, Screening fluorescent voltage indicators with spontaneously spiking HEK cells, PLoS ONE, 8, e85221, 2013.
  66. A. Fields, E. Meyer, A.E. Cohen, Euler buckling and nonlinear kinking of double-stranded DNA, Nucl. Acids Res., 41, 9881-9890, 2013.
  67. D. Maclaurin*, V. Venkatachalam*, H. Lee, A.E. Cohen, Mechanism of voltage-sensitive fluorescence in a microbial rhodopsin, PNAS, 110, 5939-5944, 2013.
  68. M. W. Elting, S.R. Leslie, L.S. Churchman, J. Korlach, C.M.J. McFaul, J.S. Leith, M.J. Levene, A.E. Cohen, J.A. Spudich, Single-molecule fluorescence imaging of processive myosin with enhanced background suppression using linear zero-mode waveguides (ZMWs) and convex lens induced confinement (CLIC), Optics Express, 21, 1189-1202, 2013.
  69. Y. Tang, L. Sun, A. E. Cohen, Chiroptical hot spots in twisted nanowire plasmonic oscillators, Applied Physics Letters, 102, 043103, 2013. Supporting Information.
  70. A. P. Fields, A. E. Cohen, Optimal tracking of a Brownian particle, Optics Express, 20, 22585-22601, 2012.
  71. M. J. Shon, A. E. Cohen, Mass action at the single-molecule level, J. Am. Chem. Soc., 134, 14618-14623, 2012. Supporting information.
  72. J. Hou, A. E. Cohen, Motion induced by asymmetric degradation of hydrogels, Soft Matter, 8, 4616-4624, 2012. Supplementary video, supplementary text.
  73. J. M. Kralj*, A. D. Douglass*, D. R. Hochbaum*, D. Maclaurin, A. E. Cohen, (*co-first authors) Optical recording of action potentials in mammalian neurons using a microbial rhodopsin, Nature Methods, 9, 90-95, 2012. Supporting Online Text and Videos.
  74. H. Lee, N. Yang, A. E. Cohen, Mapping nanomagnetic fields using a radical pair reaction, Nano Letters, 11, 5367-5372, 2011.
  75. H. Bayraktar, A. P. Fields, J. M. Kralj, J. L. Spudich, K. J. Rothschild, A. E. Cohen, Ultrasensitive measurements of microbial rhodopsin photocycles using photochromic FRET, Photochem. & Photobiol. 88, 90-97, 2012.
  76. J. M. Kralj, D. R. Hochbaum, A. D. Douglass, A. E. Cohen, Electrical spiking in Escherichia coli probed with a fluorescent voltage-indicating protein, Science, 333, 345-348, 2011. Supporting Online Text and Videos.
  77. A. E. Cohen, A. P. Fields, The cat that caught the canary: what to do with single-molecule trapping, ACS Nano, 5, 5296-5299, 2011.
  78. A. P. Fields, A. E. Cohen, Electrokinetic trapping at the one nanometer limit, PNAS, 108, 8937-8942, 2011.
  79. A. E. Cohen and W. E. Moerner, Anti-Brownian Traps, in Encyclopedia of Biophysics, G. C. K. Roberts (Ed.) (Springer, Berlin, Heidelberg, appearing 2012).
  80. Y. Tang, A. E. Cohen, Enhanced enantioselectivity in excitation of chiral molecules by superchiral light, Science, 332, 333-336, 2011. Supporting Online Material
  81. N. Yang, A. E. Cohen, Local geometry of electromagnetic fields and its role in molecular multipole transitions, J. Phys. Chem. B, 115, 5304-5311, 2011.
  82. N. Yang, A. E. Cohen, Optical imaging through scattering media via magnetically modulated fluorescence, Optics Express, 18, 25461-25467, 2010.
  83. S. R. Leslie, A. P. Fields, A. E. Cohen, Convex lens-induced confinement for imaging single molecules, Anal. Chem., 82, 6224-6229, 2010.
  84. Y. Tang, A. E. Cohen, Optical chirality and its interaction with matter, Phys. Rev. Lett (cover story), 104,163901, 2010.
  85. A. P. Fields and A. E. Cohen, Anti-Brownian traps for studies on single molecules, Methods in Enzymology, 475, 149-174, 2010.
  86. A. E. Cohen, A. P. Fields, J. H. Hou, S. Leslie, M. J. Shon, In honor of W. E. Moerner: Confining molecules for single-molecule spectroscopy, Israeli J. Chem, 49, 275-282, 2009.
  87. A. E. Cohen, Nanomagnetic control of intersystem crossing, J. Phys. Chem. A, 113, 11084-11092, 2009.
  88. N. Yang, Y. Tang, and A. E. Cohen, Spectroscopy in sculpted fields, Nano Today, 4, 269-279, 2009.
  89. Y. Tang, T. A. Cook, A. E. Cohen, Limits on fluorescence detected circular dichroism of single helicene molecules, J. Phys. Chem. A, 113, 6213-6216, 2009.
  90. P. Jain, Y. Xiao, R. Walsworth and A. E. Cohen, Surface plasmon resonance enhanced magneto-optics (SuPREMO): Faraday rotation enhancement in gold-coated iron oxide nanocrystals, NanoLetters, 9, 1644-1650, 2009.
  91. E. Tran, A. E. Cohen, R. W. Murray, M. A. Rampi and G. M. Whitesides, Redox site-mediated charge transport in a Hg−SAM//Ru(NH3)63+/2+//SAM−Hg Junction with a dynamic interelectrode separation: compatibility with redox cycling and electron hopping mechanisms, J. Am. Chem. Soc., 131, 2141-2150, 22 Jan. 2009.
  92. Y. Jiang, Q. Wang, A. E. Cohen, N. Douglas, J. Frydman, W. E. Moerner, Hardware-based anti-Brownian electrokinetic trap (ABEL trap) for single molecules: Control loop simulations and application to ATP binding stoichiometry in multi-subunit enzymes, Proc. SPIE, 7038, 703807, (2008)
  93. A. E. Cohen, W. E. Moerner: Controlling Brownian motion of single protein molecules and single fluorophores in aqueous buffer, Opt. Express, 16, 6941-6956, 12 May 2008.
  94. A. E. Cohen, W. E. Moerner: Principal components analysis of shape fluctuations of single DNA molecules, PNAS, 104, 12622-12627, 31 July 2007. Journal link and Supporting Material and Movies.
  95. A. E. Cohen, W. E. Moerner: Internal mechanical response of a polymer in solution, Phys. Rev. Lett., 98, 116001, 14 Mar. 2007. Supplementary material.
  96. A. E. Cohen and W. E. Moerner: Suppressing Brownian motion of individual biomolecules in solution, PNAS, 103, 4362-4365, 14 Mar. 2006. Journal link and Supporting Material and Movies.
  97. A. E. Cohen and W. E. Moerner: An all-glass microfluidic cell for the ABEL trap: fabrication and modeling, Proc. SPIE 5930, 2005.
  98. A. E. Cohen: Control of nanoparticles with arbitrary two-dimensional force fields, Phys. Rev. Lett., 94, 118102, 25 Mar. 2005.
  99. A. E. Cohen and W. E. Moerner: The Anti-Brownian Electrophoretic trap (ABEL Trap): tabrication and software, Proc. SPIE 5699, 2005.
  100. S. Mukamel, A. E. Cohen, and U. Harbola: Intermolecular forces and generalized TDDFT response functions in Liouville Space, Time-Dependent Density Functional Theory (eds. M. A. L. Marques, C. A. Ullrich, F. Nogueira, A. Rubio, K. Burke and E. K. U. Gross, Springer, 2006).
  101. A. E. Cohen and W. E. Moerner: Method for trapping and manipulating nanoscale objects in solution , Appl. Phys. Lett. 86, 093109, 28 Feb. 2005.
  102. A. E. Cohen and S. Mukamel: Resonant enhancement and dissipation in nonequilibrium van der Waals forces, Phys. Rev. Lett. 91, 233202, 5 Dec. 2003.
  103. A. E. Cohen: Force-extension curve of a polymer in a high-frequency electric field, Phys. Rev. Lett. 91, 235506, 5 Dec. 2003.
  104. A. E. Cohen and L. Mahadevan: Kinks, rings, and rackets in filamentous structures, PNAS, 100,12141-12146, 14 Oct. 2003.
  105. A. E. Cohen: Carbon nanotubes provide a charge, Science (letter to the editor) 300, 1235-1235, 23 May 2003.
  106. A. E. Cohen and S. Mukamel: A mechanical force accompanies fluorescence resonance energy transfer (FRET), J. Phys. Chem. A 107, 3633-3638, 15 May 2003.
  107. Holmlin RE, Haag R, Chabinyc ML, Ismagilov RF, Cohen AE, Terfort A, Rampi MA, Whitesides GM: Electron transport through thin organic films in metal-insulator-metal junctions based on self-assembled monolayers,  J. Am. Chem. Soc., 123, 5075-5085, 30 May 2001, and Supporting Material.
  108. A. E. Cohen and R. Kunz: Large-area interdigitated array microelectrodes for electrochemical sensing, Sensors and Actuators B 62, 23-29, 25 Jan 2000.
  109. J. E. Cohen, C. M. Newman, A. E. Cohen, O. L. Petchey, A. Gonzalez: Spectral mimicry: A method of synthesizing matching time series with different Fourier spectra, Circuits Systems and Signal Processing, 18, 431-442, 1999.
  110. A. E. Cohen, A. Gonzalez, J. H. Lawton, O. L. Petchey, D. Wildman, J. E. Cohen: A novel experimental apparatus to study the impact of white noise and 1/f noise on animal populations, Proc. Roy. Soc. Lond. B 265, 11-15, 7 Jan 1998.

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Theses and Dissertations:

PhD:

  1. Adam E. Cohen, Nanoscale Mechanics, Cambridge University, defended Dec. 2003
  2. Adam E. Cohen, Trapping and Manipulating Single Molecules in Solution (formatted for single-sided printing), (formatted for double-sided printing), Stanford University, defended Aug. 2006. See also errata.
  3. Yiqiao Tang, Chirality of Light and its Interaction with Chiral Matter, Harvard University, defended Nov. 2012.
  4. Nan Yang, Molecules in Sculpted Fields: Magnetic Field Effects and Multipole Transitions, Harvard University, defended Dec. 2012.
  5. Alexander P. Fields, Electrokinetic Trapping of Single Molecules, and Euler Buckling and Nonlinear Kinking of DNA, Harvard University, defended May 2013.
  6. Jennifer Hou, Dynamics in Biological Soft Materials, Harvard University, defended Oct. 2013.
  7. Min Ju Shon, Trapping and Manipulating Single Molecules of DNA, Harvard University, defended Oct. 2013.
  8. Veena Venkatachalam, Engineering Microbial Rhodopsins to Expand the Optogenetic Toolkit, Harvard University, defended May 2014.
  9. Daniel R. Hochbaum, Bringing Bioelectricity to light: all-optical electrophysiology using microbial rhodopsins, Harvard University, defended Sept. 2014.
  10. Sami Farhi, All-Optical Neurphysiology in the 1-Photon Regime, Harvard University, defended April, 2018.
  11. Vicente J. Parot, All-Optical Neurophysiology Using High-Speed Wide-Area Optical Sectioning, Harvard University, defended May, 2019.
  12. Linlin Fan, All-Optical Electrophysiology of Excitation and Inhibition in Neural Circuits, Harvard University, defended Sept, 2019.
  13. Harry McNamara, Synthetic Physiology: Manipulating and Measuring Biological Pattern Formation with Light, Harvard University, defended Sept, 2019.
  14. Phil Brooks, Methods and tool development for robust biological voltage imaging, Harvard University, defended May, 2024.

Masters

  1. Amanda B. Klaeger, All-Optical Electrophysiology in Zebrafish, Ecole Polytechnique Federale de Lausanne, defended Aug. 2019.

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Patents:

  1. A. E. Cohen, "Near-field photolithographic masks and photolithography; nanoscale patterning techniques; apparatus and method therefor," U.S. patent 5,865,978 issued February 2, 1999
  2. Charles T. Black, A. E. Cohen, Christopher B. Murray, Robert L. Sandstrom, "Scanning probe microscopy tips composed of nanoparticles and methods to form same," U.S. patent 7,282,710 issued October 16, 2007
  3. A. E. Cohen and W. E. Moerner, "Sub-micron object control arrangement and approach therefor," U.S. patent 8,057,655 issued Nov. 15, 2011
  4. A. E. Cohen, J. M. Kralj, A. D. Douglass, "Optogenetic probes for measuring membrane potential," U.S. patent 9,057,734 issued June 16, 2015
  5. A. E. Cohen, J. M. Kralj, A. D. Douglass, Daniel Hochboaum, "Systems, methods, and workflows for optogenetics analysis," U.S. patent 9,207,237 issued Dec. 8, 2015
  6. A. E. Cohen, Daniel Hochbaum, Peng Zou, Samouil Farhi, Robert Campbell, Yongxin Zhao, Daniel Jed Harrison, “Optogenetic probes for measuring membrane potential,” U.S. patent 9,518,103 issued Dec. 13 2016
  7. A. E. Cohen, J. Kralj, A. D. Douglass, D. Hochbaum, “Systems, methods, and workflows for optogenetics analysis,” U.S. patent 9,702,874 issued Jul. 11, 2017
  8. A. E. Cohen, J. Kralj, A. D. Douglass, “Optogenetic probes for measuring membrane potential,” U.S. patent 9,791,455 issued Oct. 17, 2017
  9. A. E. Cohen and Sabrina Leslie, “Convex lens-induced confinement for measuring distributions of molecular size,” U.S. patent 10,048,193 issued Aug. 14, 2018
  10. A. E. Cohen, M. P. Chien, “Optical selection of cells,” U.S. patent 10,077,463 issued Sept. 18, 2018
  11. A. E. Cohen, J. Kralj, A. D. Douglass, D. R. Hochbaum, “Systems, methods and workflows for optogenetics analysis,” U.S. patent 10,161,937 issued 25 Dec. 2018
  12. A. E. Cohen, J. Kralj, A. D. Douglass, “Optogenetic probes for measuring membrane potential,” U.S. patent 10,352,945 issued Jul. 16, 2019
  13. A. E. Cohen, Daniel Hochbaum, Peng Zou, Samouil Farhi, Robert Campbell, Yongxin Zhao, Daniel Jed Harrison, “Optogenetic probes for measuring membrane potential,” U.S. patent 10,457,715 issued Oct. 29, 2019
  14. A. E. Cohen, J. H. Hou, “Improvement to spinning disk microscopy”, U.S. patent pending

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Book:

  1. Let's Go 2000: Peru and Ecuador (Here's a link to the current website)

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Contact:

tel: (617) 496-9466
email: cohen@chemistry.harvard.edu
Office: Mallinckrodt 115

 

Harvard University
12 Oxford Street
Cambridge, MA 02138

Administrator: Tracey Schaal

tel: (617) 496-8233
email: schaal@chemistry.harvard.edu